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ProductsBack/CD45 Polyclonal Antibody
Raji cells probed with \tCD45 Polyclonal Antibody, Unconjugated (bs-0522R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Raji cells probed with \tCD45 Polyclonal Antibody, Unconjugated (bs-0522R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Raji cells probed with \tCD45 Polyclonal Antibody, Unconjugated (bs-0522R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

CD45 Polyclonal Antibody

BS-0522R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPTPRC
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD45 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5788
  • Target name
    PTPRC
  • Target description
    protein tyrosine phosphatase receptor type C
  • Target synonyms
    B220; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08575
  • Protein Name
    Receptor-type tyrosine-protein phosphatase C
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte. Jin J et al., 2019 Mar 15, Stem Cell Res Ther
    Read more
  • Ecdysterone Accelerates Healing of Radiation-Induced Oral Mucositis in Rats by Increasing Matrix Cell Proliferation. Li Y et al., 2019 Mar, Radiat Res
    Read more
  • Expression of Pref-1 and Related Chemokines during theDevelopment of Rat Mesenteric Lymph Nodes. Peng Y et al., 2018 Jul, Biomed Environ Sci
    Read more
  • Bone mesenchymal stem cells co-expressing VEGF and BMP-6 genes to combat avascular necrosis of the femoral head. Liao H et al., 2018 Jan, Exp Ther Med
    Read more
  • Effects of neuritin on the differentiation of bone marrow-derived mesenchymal stem cells into neuron-like cells. Zhu J et al., 2017 Sep, Mol Med Rep
    Read more
  • Common expression of stemness molecular markers and early cardiac transcription factors in human Whartons jelly-derived mesenchymal stem cells and embryonic stem cells. Gao LR et al., 2013, Cell Transplant
    Read more
  • Increased expression of importin13 in endometriosis and endometrial carcinoma. Zeng B et al., 2012 Jun, Med Sci Monit
    Read more

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Figure 1. Western blot analysis of CD45/PTPRC using anti-CD45/PTPRC antibody (A00555-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45/PTPRC antigen affinity purified polyclonal antibody (Catalog # A00555-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45/PTPRC at approximately 220 kDa. The expected band size for CD45/PTPRC is at 220 kDa.
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