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CD49C Antibody

HY-P84216
MedChem Express
TargetITGA3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CD49C Antibody
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3675
  • Target name
    ITGA3
  • Target description
    integrin subunit alpha 3
  • Target synonyms
    alpha 3 subunit of VLA-3 receptor; antigen CD49C; antigen identified by monoclonal antibody J143; CD49 antigen-like family member C; CD49C; FRP-2; galactoprotein B3; GAPB3; GAP-B3; ILNEB; integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); MSK18; testicular tissue protein Li 96; testicular tissue protein Li 98; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA3a
  • Host
    Mouse
  • Isotype
    IgG1
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 3/ITGA3 using anti-Integrin Alpha 3/ITGA3 antibody (A02902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 3/ITGA3 antigen affinity purified polyclonal antibody (Catalog # A02902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 3/ITGA3 at approximately 130 kDa. The expected band size for Integrin Alpha 3/ITGA3 is at 130 kDa.
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