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Western Blot analysis of HepG2 cells using Integrin alpha3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Integrin alpha3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Integrin alpha3 Polyclonal Antibody

ITGA3 Antibody

CSB-PA003045
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetITGA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITGA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3675
  • Target name
    ITGA3
  • Target description
    integrin subunit alpha 3
  • Target synonyms
    CD49C, FRP-2, GAP-B3, GAPB3, ILNEB, JEB7, MSK18, VCA-2, VL3A, VLA3a, integrin alpha-3, CD49 antigen-like family member C, alpha 3 subunit of VLA-3 receptor, antigen CD49C, antigen identified by monoclonal antibody J143, galactoprotein B3, integrin alpha 3, integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor), testicular tissue protein Li 96, testicular tissue protein Li 98, very late activation protein 3 receptor, alpha-3 subunit, very late activation receptor subunit alpha-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26006
  • Protein Name
    Integrin alpha-3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin Alpha 3/ITGA3 using anti-Integrin Alpha 3/ITGA3 antibody (A02902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 3/ITGA3 antigen affinity purified polyclonal antibody (Catalog # A02902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 3/ITGA3 at approximately 130 kDa. The expected band size for Integrin Alpha 3/ITGA3 is at 130 kDa.
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