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ProductsBack/CD5 antibody [MRC OX-19] (Biotin)

CD5 antibody [MRC OX-19] (Biotin)

GTX76267
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityRat
TargetCd5
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD5 antibody [MRC OX-19] (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    For FACS: Use 0.1 ug to label 10^6 cells. Protocols are available upon request. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MRC OX-19
  • Concentration
    0.1 mg/ml
  • Conjugate
    Biotin
  • Gene ID54236
  • Target name
    Cd5
  • Target description
    Cd5 molecule
  • Target synonyms
    T-cell surface glycoprotein CD5, CD5 antigen, Lymphocyte antigen CD5, T-cell surface glycoprotein CD5-like
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51882
  • Protein Name
    T-cell surface glycoprotein CD5
  • Scientific Description
    binds CD2 glycoprotein on thymocytes; may play a role in T lymphocyte signal transduction [RGD, Feb 2006]
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (M00480-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD5 antigen affinity purified monoclonal antibody (Catalog # M00480-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 67KD
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