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ProductsBack/Cd5 Rat Monoclonal Antibody [Clone ID: 4H8E6]
Immunofluorescent Staining: Cells from BALB/c spleen were double-stained with Rat anti-Mouse CD5-R-PE and rat anti-mouse CD19-FITC (Clone 6D5), following which small lymphocytes were gated and analyzed using a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount used: < / = 0.2 ug/10e6 cells.
Immunofluorescent Staining: Cells from BALB/c spleen were double-stained with Rat anti-Mouse CD5-R-PE and rat anti-mouse CD19-FITC (Clone 6D5), following which small lymphocytes were gated and analyzed using a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount used: &lt; / = 0.2 ug/10e6 cells.
Immunofluorescent Staining: Cells from BALB/c spleen were double-stained with Rat anti-Mouse CD5-R-PE and rat anti-mouse CD19-FITC (Clone 6D5), following which small lymphocytes were gated and analyzed using a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount used: < / = 0.2 ug/10e6 cells.

Cd5 Rat Monoclonal Antibody [Clone ID: 4H8E6]

AM08024RP-N
OriGene
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCd5
Price on request
200 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cd5 Rat Monoclonal Antibody [Clone ID: 4H8E6]
  • Delivery Days Customer
    14
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4H8E6
  • Gene ID12507
  • Target name
    Cd5
  • Target description
    CD5 antigen
  • Target synonyms
    Ly-1, Ly-12, Ly-A, Lyt-1, T-cell surface glycoprotein CD5, lymphocyte antigen 1
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP13379
  • Protein Name
    T-cell surface glycoprotein CD5
  • Scientific Description
    Cd5 rat monoclonal antibody, clone 4H8E6, PE
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (A00480-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 70 kDa. The expected band size for CD5 is at 54 kDa.
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