CD53(161-2), CF740 conjugate, 0.1mg/mL

Biotium

CD53(161-2), CF740 conjugate, 0.1mg/mL

16

Functional Assay

Research Use Only

Monoclonal

161-2

0.1 mg/ml

Other Conjugate

CD53

CD53 molecule

MOX44, TSPAN25, leukocyte surface antigen CD53, CD53 antigen, CD53 glycoprotein, CD53 tetraspan antigen, antigen MOX44 identified by monoclonal antibody MRC-OX44, cell surface antigen, cell surface glycoprotein CD53, tetraspanin-25, transmembrane glycoprotein, tspan-25

IgG2a

Leukocyte surface antigen CD53

Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Animal

2°C to 8°C,RT

41116100