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ProductsBack/CD55 antibody [Bu14] (FITC)
FACS analysis of human peripheral blood monocytes using GTX43536 CD55 antibody [Bu14] (FITC).
FACS analysis of human peripheral blood monocytes using GTX43536 CD55 antibody [Bu14] (FITC).
FACS analysis of human peripheral blood monocytes using GTX43536 CD55 antibody [Bu14] (FITC).

CD55 antibody [Bu14] (FITC)

GTX43536
GeneTex
ApplicationsFlow Cytometry
DownloadDatasheet
Product group Antibodies
TargetCD55
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD55 antibody [Bu14] (FITC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: Neat-1/10. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Bu14
  • Concentration
    0.1 mg/ml
  • Conjugate
    FITC
  • Gene ID1604
  • Target name
    CD55
  • Target description
    CD55 molecule (Cromer blood group)
  • Target synonyms
    CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CHAPLE; complement decay-accelerating factor; CR; CROM; Cromer blood group antigen; DAF; Rh blood group D antigen; TC
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08174
  • Protein Name
    Complement decay-accelerating factor
  • Scientific Description
    This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (PB9487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD55 antigen affinity purified polyclonal antibody (Catalog # PB9487) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD55 at approximately 75KD. The expected band size for CD55 is at 75KD.
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