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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA004945LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA004945LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA004945LA01HU at dilution of 1:100

CD55 Antibody

CSB-PA004945LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD55
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD55 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1604
  • Target name
    CD55
  • Target description
    CD55 molecule (Cromer blood group)
  • Target synonyms
    CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CHAPLE; complement decay-accelerating factor; CR; CROM; Cromer blood group antigen; DAF; Rh blood group D antigen; TC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08174
  • Protein Name
    Complement decay-accelerating factor
  • Scientific Description
    This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (PB9487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD55 antigen affinity purified polyclonal antibody (Catalog # PB9487) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD55 at approximately 75KD. The expected band size for CD55 is at 75KD.
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