FACS analysis of BAF-3 cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
![FACS analysis of PBMC cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue). Dilution : 0.5 ug/test incubated for 30 min at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15669/GTX15669_103_FACS_w_23060620_708.webp "FACS analysis of PBMC cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue). Dilution : 0.5 ug/test incubated for 30 min at 4oC")
![WB analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) using GTX15669 CD58 antibody [TS2/9]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX15669/GTX15669_1514_WB_w_23060620_600.webp "WB analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) using GTX15669 CD58 antibody [TS2/9]. Dilution : 1:100")
![ICC/IF analysis of Raji cells using GTX15669 CD58 antibody [TS2/9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15669/GTX15669_285_ICC-IF_w_23060620_340.webp "ICC/IF analysis of Raji cells using GTX15669 CD58 antibody [TS2/9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes Dilution : 1:20 overnight at 4oC")
FACS analysis of BAF-3 cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
CD58 antibody [TS2/9]
GTX15669
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman, Mouse
TargetCD58
Overview
- SupplierGeneTex
- Product NameCD58 antibody [TS2/9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDTS2/9
- Concentration0.98 mg/ml
- ConjugateUnconjugated
- Gene ID965
- Target nameCD58
- Target descriptionCD58 molecule
- Target synonymsag3; CD58 antigen, (lymphocyte function-associated antigen 3); LFA3; LFA-3; lymphocyte function-associated antigen 3; surface glycoprotein LFA-3
- HostMouse
- IsotypeIgG1
- Protein IDP19256
- Protein NameLymphocyte function-associated antigen 3
- Scientific DescriptionThis gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203