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Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.

CD62p Polyclonal Antibody

BS-0561R
Bioss
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSelp
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Overview

  • Supplier
    Bioss
  • Product Name
    CD62p Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(1:100-500), IHC-F(1:100-500), IF(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID20344
  • Target name
    Selp
  • Target description
    selectin, platelet
  • Target synonyms
    CD62P, GMP-140, Grmp, LECAM3, PADGEM, P-selectin, CD62 antigen-like family member P, granule membrane protein 140, leukocyte-endothelial cell adhesion molecule 3, platelet activation dependent granule-external membrane protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01102
  • Protein Name
    P-selectin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Rui-Kai Z, Jian L. Effects of xinfeng capsules on expression of platelet granule membrane protein 140 and platelet cluster of differentiation 40 ligand in peripheral blood of adjuvant arthritis rats. Int J Rheumatol. 2012,2012:139696. doi: 10.1155/2012/139696
    Read this paper

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Figure 1. Western blot analysis of CD62P using anti-CD62P antibody (A01241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody (Catalog # A01241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62P at approximately 110KD. The expected band size for CD62P is at 91KD.
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