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ProductsBack/CD71 antibody [3B8 2A1]

CD71 antibody [3B8 2A1]

GTX54454
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetTFRC
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD71 antibody [3B8 2A1]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B8 2A1
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID7037
  • Target name
    TFRC
  • Target description
    transferrin receptor
  • Target synonyms
    CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90, transferrin receptor protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02786
  • Protein Name
    Transferrin receptor protein 1
  • Scientific Description
    This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TFRC using anti-TFRC antibody (PB9233). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat RAW264.7 whole cell lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFRC antigen affinity purified polyclonal antibody (Catalog # PB9233) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFRC at approximately 95 kDa. The expected band size for TFRC is at 85 kDa.
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