Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:1000.
![CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD71 protein stained by CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102596/GTX102596_42025_20150831_IFA_w_23060119_757.webp "CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD71 protein stained by CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Wild-type (WT) and CD71 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102596/GTX102596_42025_20181123_WB_KO_watermark_w_23060119_494.webp "Wild-type (WT) and CD71 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102596/GTX102596_42319_20220107_WB_M_w_23060119_499.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:1000 and competitor's antibody (sc-32272) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102596/GTX102596_42319_20171124_WB_competitor_w_23060119_395.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:1000 and competitor's antibody (sc-32272) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:1000.
CD71 antibody [N2C1], Internal
GTX102596
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTFRC
Overview
- SupplierGeneTex
- Product NameCD71 antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.47 mg/ml
- ConjugateUnconjugated
- Gene ID7037
- Target nameTFRC
- Target descriptiontransferrin receptor
- Target synonymsCD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90, transferrin receptor protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP02786
- Protein NameTransferrin receptor protein 1
- Scientific DescriptionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Tsai LT, Weng TI, Chang TY, et al. Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo. Antioxidants (Basel). 2023,12(11). doi: 10.3390/antiox12111931Read this paper
- Karahoda R, Zaugg J, Fuenzalida B, et al. Trophoblast Differentiation Affects Crucial Nutritive Functions of Placental Membrane Transporters. Front Cell Dev Biol. 2022,10:820286. doi: 10.3389/fcell.2022.820286Read this paper
- Singh KS, Leu JI, Barnoud T, et al. African-centric TP53 variant increases iron accumulation and bacterial pathogenesis but improves response to malaria toxin. Nat Commun. 2020,11(1):473. doi: 10.1038/s41467-019-14151-9Read this paper
Datasheet
Related products
Product group Antibodies
Anti-CD71 [Q1/71]Ab01409-1.1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTFRC
- SizePrice
Product group Antibodies
ApplicationsFlow Cytometry
TargetTFRC
- SizePrice
Product group Antibodies
ApplicationsFlow Cytometry
ReactivityHuman
TargetTFRC
- SizePrice
Product group Antibodies
References
CD71 antibody [MEM-75] (Biotin)GTX78311
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry
ReactivityHuman
TargetTFRC
- SizePrice
![FACS analysis of K562 cells (positive) and lymphocytes (negative) using GTX78312 CD71 antibody [MEM-75] (FITC).](https://www.genetex.com/upload/website/prouct_img/normal/GTX78312/GTX78312_20191025_AP_006_288_w_23061322_228.webp)
Product group Antibodies
References
CD71 antibody [MEM-75] (FITC)GTX78312
ApplicationsFlow Cytometry
ReactivityHuman, Rabbit
TargetTFRC
- SizePrice
![FACS analysis of K562 cells (red) and human lymphocytes (negative, grey) using GTX79925 CD71 antibody [MEM-75] (APC).](https://www.genetex.com/upload/website/prouct_img/normal/GTX79925/GTX79925_20191025_AP_006_332_w_23061322_138.webp)
Product group Antibodies
References
CD71 antibody [MEM-75] (APC)GTX79925
ApplicationsFlow Cytometry
ReactivityHuman
TargetTFRC
- SizePrice
Product group Antibodies
Tfrc Polyclonal AntibodyCAC07319
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetTFRC
- SizePrice
Product group Antibodies
TFRC Polyclonal AntibodyBS-0988R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetTFRC
- SizePrice
