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ProductsBack/CD72 antibody [4G10]

CD72 antibody [4G10]

GTX53282
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCd72
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD72 antibody [4G10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4G10
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID12517
  • Target name
    Cd72
  • Target description
    CD72 antigen
  • Target synonyms
    CD72c, Ly-19, Ly-32, Ly-m19, Lyb-2, B-cell differentiation antigen CD72, lymphocyte antigen 32
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP21855
  • Protein Name
    B-cell differentiation antigen CD72
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD72 using anti-CD72 antibody (A09292-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD72 antigen affinity purified polyclonal antibody (Catalog # A09292-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD72 at approximately 35KD. The expected band size for CD72 is at 35KD.
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