Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638851/GTX638851_T-45110_20230721_WB_M_R_23072519_495.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638851/GTX638851_45173_20230922_WB_Z_23100118_253.webp "Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CD73 antibody [HL2493] detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD73 stained by CD73 antibody [HL2493] (GTX638851) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638851/GTX638851_45173_20231020_IHC-P_23102401_165.webp "CD73 antibody [HL2493] detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD73 stained by CD73 antibody [HL2493] (GTX638851) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638851/GTX638851_45173_20231006_WB_TPM_watermark_23102401_597.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CD73 antibody [HL2493]
GTX638851
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetNT5E
Overview
- SupplierGeneTex
- Product NameCD73 antibody [HL2493]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2493
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4907
- Target nameNT5E
- Target description5'-nucleotidase ecto
- Target synonymsCALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, IMP-specific 5'-nucleotidase, Purine 5-Prime-Nucleotidase, ecto-5'-nucleotidase, thymidylate 5'-phosphatase
- HostRabbit
- IsotypeIgG
- Protein IDP21589
- Protein Name5'-nucleotidase
- Scientific DescriptionThe protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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