Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110477/GTX110477_44475_20211119_IHC-P_w_23060500_260.webp "CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110477/GTX110477_44545_20220128_IHC-P_w_23060500_572.webp "CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110477/GTX110477_44545_20220128_IHC-P_1_w_23060500_609.webp "CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CD74 antibody [N1N2], N-term detects CD74 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX110477/GTX110477_44580_20220916_ICC_IF_23100319_446.webp "CD74 antibody [N1N2], N-term detects CD74 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110477/GTX110477_45189_20231013_WB_TPM_watermark_24112622_273.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110477/GTX110477_44475_20250110_WB_R_spleen_25011618_603.webp "Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CD74 antibody [N1N2], N-term
GTX110477
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCD74
Overview
- SupplierGeneTex
- Product NameCD74 antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.45 mg/ml
- ConjugateUnconjugated
- Gene ID972
- Target nameCD74
- Target descriptionCD74 molecule
- Target synonymsCLIP, DHLAG, HLADG, II, Ia-GAMMA, p33, HLA class II histocompatibility antigen gamma chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), CD74 molecule, major histocompatibility complex, class II invariant chain, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma chain, class II MHC-associated invariant chain peptide, gamma chain of class II antigens
- HostRabbit
- IsotypeIgG
- Protein IDP04233
- Protein NameHLA class II histocompatibility antigen gamma chain
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sreevalsan S, Döring M, Paszkowski-Rogacz M, et al. MLLT6 maintains PD-L1 expression and mediates tumor immune resistance. EMBO Rep. 2020,21(12):e50155. doi: 10.15252/embr.202050155Read this paper
- Liu YH, Shen CY, Tsai FJ. Association of variant on the promoter of cluster of differentiation 74 in graves disease and graves ophthalmopathy. Biosci Rep. 2020,40(8). doi: 10.1042/BSR20202072Read this paper
- Guest JD, Moore SW, Aimetti AA, et al. Internal decompression of the acutely contused spinal cord: Differential effects of irrigation only versus biodegradable scaffold implantation. Biomaterials. 2018,185:284-300. doi: 10.1016/j.biomaterials.2018.09.025Read this paper
- Chen YT, Chiang HJ, Chen CH, et al. Melatonin treatment further improves adipose-derived mesenchymal stem cell therapy for acute interstitial cystitis in rat. J Pineal Res. 2014,57(3):248-61. doi: 10.1111/jpi.12164Read this paper
Datasheet
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