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ProductsBack/CD79a antibody [HM57] (PE)
Intracellular staining of CD79a with anti-CD79a (HM57) PE gated on leukemic blast cells) in a patient with childhood B-precursor ALL.
Intracellular staining of CD79a with anti-CD79a (HM57) PE gated on leukemic blast cells) in a patient with childhood B-precursor ALL.
Intracellular staining of CD79a with anti-CD79a (HM57) PE gated on leukemic blast cells) in a patient with childhood B-precursor ALL.

CD79a antibody [HM57] (PE)

GTX80094
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityBovine, Chicken, Equine, Guinea Pig, Human, Mouse, Opossum, Porcine, Rabbit, Rat
TargetCD79A
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD79a antibody [HM57] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 10 microl reagent / 100 microl of whole blood or 106 cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HM57
  • Conjugate
    RPE
  • Gene ID973
  • Target name
    CD79A
  • Target description
    CD79a molecule
  • Target synonyms
    IGA, IGAlpha, MB-1, MB1, B-cell antigen receptor complex-associated protein alpha chain, CD79a antigen (immunoglobulin-associated alpha), CD79a molecule, immunoglobulin-associated alpha, MB-1 membrane glycoprotein, ig-alpha, membrane-bound immunoglobulin-associated protein, surface IgM-associated protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11912
  • Protein Name
    B-cell antigen receptor complex-associated protein alpha chain
  • Scientific Description
    The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  • Reactivity
    Bovine, Chicken, Equine, Guinea Pig, Human, Mouse, Opossum, Porcine, Rabbit, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Moreira AG, Siddiqui SK, Macias R, et al. Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells. PLoS One. 2020,15(3):e0229521. doi: 10.1371/journal.pone.0229521
    Read this paper

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Figure 1. Western blot analysis of CD79A using anti-CD79A antibody (PB9387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79A antigen affinity purified polyclonal antibody (Catalog # PB9387) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD79A at approximately 44 kDa. The expected band size for CD79A is at 25 kDa.
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