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ProductsBack/CD8 alpha antibody [MAB0708]
IHC-P analysis of formalin fixed mouse tumor tissue section using GTX53126 CD8 alpha antibody [MAB0708].
IHC-P analysis of formalin fixed mouse tumor tissue section using GTX53126 CD8 alpha antibody [MAB0708].
IHC-P analysis of formalin fixed mouse tumor tissue section using GTX53126 CD8 alpha antibody [MAB0708].

CD8 alpha antibody [MAB0708]

GTX53126
GeneTex
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetCd8a
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD8 alpha antibody [MAB0708]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100 - 1:300. FACS: 1:100 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MAB0708
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID12525
  • Target name
    Cd8a
  • Target description
    CD8 subunit alpha
  • Target synonyms
    Ly-2, Ly-35, Ly-B, Lyt-2, T-cell surface glycoprotein CD8 alpha chain, CD8 antigen, alpha chain, Lyt-2.1 lymphocyte differentiation antigen (AA at 100), T-cell surface glycoprotein Lyt-2
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP01731
  • Protein Name
    T-cell surface glycoprotein CD8 alpha chain
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Huang CP, Wu CC, Shyr CR. Combination of novel intravesical xenogeneic urothelial cell immunotherapy and chemotherapy enhances anti-tumor efficacy in preclinical murine bladder tumor models. Cancer Immunol Immunother. 2021,70(5):1419-1433. doi: 10.1007/s00262-020-02775-6
    Read this paper
  • Song DJ, Miller M, Beppu A, et al. Rhinovirus Infection of ORMDL3 Transgenic Mice Is Associated with Reduced Rhinovirus Viral Load and Airway Inflammation. J Immunol. 2017,199(7):2215-2224. doi: 10.4049/jimmunol.1601412
    Read this paper

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Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody (Catalog # A02236-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD.
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