CD8+ T cells, Negatively Selected (Human)
79753
Product group (Cell) Culture
Overview
- SupplierBPS Bioscience
- Product NameCD8+ T cells, Negatively Selected (Human)
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionCryopreserved vial (10 x 10^6 cells) of CD8+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD4+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD8+ T cells via immunomagnetic separation. Before and after CD8+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate. Source Normal human PBMC from Leukapheresis Sample
- UNSPSC12352207