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ProductsBack/CD81 Antibody - middle region (OASG01356)

CD81 Antibody - middle region (OASG01356)

OASG01356
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetCd81
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CD81 Antibody - middle region (OASG01356)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID12520
  • Target name
    Cd81
  • Target description
    CD81 antigen
  • Target synonyms
    Tapa-1, Tapa1, Tspan28, CD81 antigen, 26 kDa cell surface protein TAPA-1, target of the antiproliferative antibody 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAPA1/CD81 using anti-TAPA1/CD81 antibody (A01281-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1/CD81 antigen affinity purified polyclonal antibody (Catalog # A01281-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1/CD81 at approximately 22 kDa. The expected band size for TAPA1/CD81 is at 26 kDa.
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