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ProductsBack/CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: 2F7]

CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: 2F7]

AM08071LE-N
OriGene
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCd81
Price on request
500 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: 2F7]
  • Delivery Days Customer
    14
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F7
  • Gene ID12520
  • Target name
    Cd81
  • Target description
    CD81 antigen
  • Target synonyms
    Tapa-1, Tapa1, Tspan28, CD81 antigen, 26 kDa cell surface protein TAPA-1, target of the antiproliferative antibody 1
  • Host
    Hamster
  • Isotype
    IgG
  • Protein IDP35762
  • Protein Name
    CD81 antigen
  • Scientific Description
    CD81 / TAPA1 hamster monoclonal antibody, clone 2F7, Low Endotoxin
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAPA1/CD81 using anti-TAPA1/CD81 antibody (A01281-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1/CD81 antigen affinity purified polyclonal antibody (Catalog # A01281-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1/CD81 at approximately 22 kDa. The expected band size for TAPA1/CD81 is at 26 kDa.
Product group Antibodies
Boster Bio
Anti-TAPA1/Cd81 Antibody Picoband(r)A01281-3-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCd81
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Cusabio
Cd81 AntibodyCSB-PA004960DA01MO
ApplicationsELISA
ReactivityMouse
TargetCd81
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