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ProductsBack/CD82 Antibody (C-Terminus, Magnetic beads)

CD82 Antibody (C-Terminus, Magnetic beads)

LS-C172106
LifeSpan BioSciences
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetCD82
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD82 Antibody (C-Terminus, Magnetic beads)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation
  • Applications Supplier
    IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    BioMagnetic Particle
  • Estimated Purity
    ...
  • Gene ID3732
  • Target name
    CD82
  • Target description
    CD82 molecule
  • Target synonyms
    4F9; C33; C33 antigen; CD82 antigen; GR15; IA4; inducible membrane protein R2; KAI1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)); metastasis suppressor Kangai-1; R2; SAR2; ST6; tetraspanin-27; TSPAN27; tspan-27
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD82 using anti-CD82 antibody (PB9170). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD82 antigen affinity purified polyclonal antibody (Catalog # PB9170) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 30-36 kDa. The expected band size for CD82 is at 30 kDa.
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IHC image of CSB-PA13269A0Rb diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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