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ProductsBack/CD82 Recombinant Antibody, AbBy Fluor-488 Conjugated

CD82 Recombinant Antibody, AbBy Fluor-488 Conjugated

BSM-61627R-BF488
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetCD82
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Overview

  • Supplier
    Bioss
  • Product Name
    CD82 Recombinant Antibody, AbBy Fluor-488 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID3732
  • Target name
    CD82
  • Target description
    CD82 molecule
  • Target synonyms
    4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27, CD82 antigen, C33 antigen, inducible membrane protein R2, kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)), metastasis suppressor Kangai-1, tetraspanin-27, tspan-27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27701
  • Protein Name
    CD82 antigen
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD82 using anti-CD82 antibody (PB9170). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD82 antigen affinity purified polyclonal antibody (Catalog # PB9170) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 30-36 kDa. The expected band size for CD82 is at 30 kDa.
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