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ProductsBack/CD88 / C5R1 Rat Monoclonal Antibody [Clone ID: 20/70]

CD88 / C5R1 Rat Monoclonal Antibody [Clone ID: 20/70]

AM01270RP-N
OriGene
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetC5ar1
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100 tests
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Overview

  • Supplier
    OriGene
  • Product Name
    CD88 / C5R1 Rat Monoclonal Antibody [Clone ID: 20/70]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    20/70
  • Gene ID12273
  • Target name
    C5ar1
  • Target description
    complement component 5a receptor 1
  • Target synonyms
    C5aR, C5r1, Cd88, D7Msu1, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5a ligand, C5a-R, complement component 5, receptor 1
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP30993
  • Protein Name
    C5a anaphylatoxin chemotactic receptor 1
  • Scientific Description
    CD88 / C5R1 rat monoclonal antibody, clone 20/70, PE
  • Reactivity
    Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C5a-R/C5ar1 using anti-C5a-R/C5ar1 antibody (A01898-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5a-R/C5ar1 antigen affinity purified polyclonal antibody (Catalog # A01898-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5a-R/C5ar1 at approximately 43KD. The expected band size for C5a-R/C5ar1 is at 43KD.
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ReactivityMouse
TargetC5ar1
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ReactivityMouse
TargetC5ar1
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