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CDC123 Antibody

LS-C829941
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCDC123
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDC123 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8872
  • Target name
    CDC123
  • Target description
    cell division cycle 123
  • Target synonyms
    C10orf7, D123, translation initiation factor eIF2 assembly protein, HT-1080, PZ32, cell division cycle 123 homolog, cell division cycle protein 123 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDC123 using anti-CDC123 antibody (A08251-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC123 antigen affinity purified polyclonal antibody (Catalog # A08251-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC123 at approximately 45KD. The expected band size for CDC123 is at 45KD.
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