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CDC20 Antibody (aa57-84)

LS-C168583
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetCDC20
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDC20 Antibody (aa57-84)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo (1:10 - 1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID991
  • Target name
    CDC20
  • Target description
    cell division cycle 20
  • Target synonyms
    bA276H19.3; CDC20 cell division cycle 20 homolog; CDC20A; cell division cycle 20 homolog; cell division cycle protein 20 homolog; fizzy homolog; p55CDC
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdc20 using anti-Cdc20 antibody (A00382-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc20 antigen affinity purified polyclonal antibody (Catalog # A00382-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc20 at approximately 55KD. The expected band size for Cdc20 is at 55KD.
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