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Western Blot analysis of 3T3 cells using Cdc20 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Cdc20 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Cdc20 Polyclonal Antibody

CDC20 Antibody

CSB-PA001497
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDC20
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC20 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID991
  • Target name
    CDC20
  • Target description
    cell division cycle 20
  • Target synonyms
    CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC, cell division cycle protein 20 homolog, CDC20 cell division cycle 20 homolog, cell division cycle 20 homolog, fizzy homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12834
  • Protein Name
    Cell division cycle protein 20 homolog
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cdc20 using anti-Cdc20 antibody (A00382-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc20 antigen affinity purified polyclonal antibody (Catalog # A00382-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc20 at approximately 55KD. The expected band size for Cdc20 is at 55KD.
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