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WB analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody. Dilution : 1:1000 Loading : 10 microg
WB analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody. Dilution : 1:1000 Loading : 10 microg
WB analysis of HeLa whole cell lysate using GTX00896 CDC6 antibody. Dilution : 1:1000 Loading : 10 microg

CDC6 antibody

GTX00896
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCDC6
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Overview

  • Supplier
    GeneTex
  • Product Name
    CDC6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:3000. ICC/IF: 1:200. IP: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID990
  • Target name
    CDC6
  • Target description
    cell division cycle 6
  • Target synonyms
    CDC18L; cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; cell division control protein 6 homolog; cell division cycle 6 homolog; HsCDC18; HsCDC6; MGORS5; p62(cdc6)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99741
  • Protein Name
    Cell division control protein 6 homolog
  • Scientific Description
    The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdc6 using anti-Cdc6 antibody (PB9531). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: JURKAT Whole Cell Lysate at 40ug, Lane 3: MM231 Whole Cell Lysate at 40ug, Lane 4: HT1080 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc6 antigen affinity purified polyclonal antibody (Catalog # PB9531) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc6 at approximately 63 kDa. The expected band size for Cdc6 is at 63 kDa.
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