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Western blot All lanes: CDC7 antibody at 0.95ug/ml + SY-5Y whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa
Western blot All lanes: CDC7 antibody at 0.95ug/ml + SY-5Y whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa
Western blot All lanes: CDC7 antibody at 0.95ug/ml + SY-5Y whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa

CDC7 Antibody

CSB-PA005023ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDC7
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDC7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8317
  • Target name
    CDC7
  • Target description
    cell division cycle 7
  • Target synonyms
    CDC7L1, HsCDC7, Hsk1, huCDC7, cell division cycle 7-related protein kinase, CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1, CDC7-related kinase, cell division cycle 7 homolog, cell division cycle 7-like protein 1, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00311
  • Protein Name
    Cell division cycle 7-related protein kinase
  • Scientific Description
    Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC7 using anti-CDC7 antibody (A01190-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC7 antigen affinity purified polyclonal antibody (Catalog # A01190-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC7 at approximately 64 kDa. The expected band size for CDC7 is at 64 kDa.
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