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ProductsBack/CDH4 / R Cadherin Antibody

CDH4 / R Cadherin Antibody

LS-C497267
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCDH4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDH4 / R Cadherin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 92kDa/100kDa, while the observed MW by Western blot was 100kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 92kDa/100kDa, while the observed MW by Western blot was 100kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1002
  • Target name
    CDH4
  • Target description
    cadherin 4
  • Target synonyms
    CAD4, R-CAD, RCAD, cadherin-4, R-cadherin, cadherin 4, type 1, R-cadherin (retinal), cadherin 4, type 1, preproprotein, retinal cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody (A07632-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-R Cadherin/CDH4 antigen affinity purified polyclonal antibody (Catalog # A07632-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for R Cadherin/CDH4 at approximately 125KD. The expected band size for R Cadherin/CDH4 is at 125KD.
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