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ProductsBack/CDK12 / CRKRS Antibody

CDK12 / CRKRS Antibody

LS-C749874
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDK12 / CRKRS Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 141kDa/163kDa/164kDa, while the observed MW by Western blot was 164kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 141kDa/163kDa/164kDa, while the observed MW by Western blot was 164kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    4.11 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51755
  • Target name
    CDK12
  • Target description
    cyclin dependent kinase 12
  • Target synonyms
    CRK7, CRKR, CRKRS, cyclin-dependent kinase 12, CDC2-related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, cell division cycle 2-related protein kinase 7, cell division protein kinase 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDK12 using anti-CDK12 antibody (A03495-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK12 antigen affinity purified polyclonal antibody (Catalog # A03495-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK12 at approximately 205 kDa. The expected band size for CDK12 is at 164 kDa.
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