Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5(GTX108328) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody [C2C3], C-term (GTX108328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_20180209_WB_KO_watermark_w_23060120_127.webp "Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody [C2C3], C-term (GTX108328) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. CDK5 antibody [C2C3], C-term (GTX108328) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX108328/GTX108328_39750_IHC_R_w_23060120_736.webp "CDK5 antibody [C2C3], C-term detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. CDK5 antibody [C2C3], C-term (GTX108328) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5(GTX108328) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
CDK5 antibody [C2C3], C-term
GTX108328
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK5
Overview
- SupplierGeneTex
- Product NameCDK5 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1020
- Target nameCDK5
- Target descriptioncyclin dependent kinase 5
- Target synonymsLIS7, PSSALRE, cyclin-dependent kinase 5, TPKII catalytic subunit, cell division protein kinase 5, cyclin-dependent-like kinase 5, epididymis secretory sperm binding protein, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit
- HostRabbit
- IsotypeIgG
- Protein IDQ00535
- Protein NameCyclin-dependent kinase 5
- Scientific DescriptionProbably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhong Y, Chen J, et al. Crosstalk between Cdk5/p35 and ERK1/2 signalling mediates spinal astrocyte activity via the PPARγ pathway in a rat model of chronic constriction injury. J Neurochem. 2019,151(2):166-184. doi: 10.1111/jnc.14827Read this paper
Datasheet
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