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Western Blot analysis of 293 cells using Cdk8 Polyclonal Antibody
Western Blot analysis of 293 cells using Cdk8 Polyclonal Antibody
Western Blot analysis of 293 cells using Cdk8 Polyclonal Antibody

CDK8 Antibody

CSB-PA001541
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCDK8
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDK8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1024
  • Target name
    CDK8
  • Target description
    cyclin dependent kinase 8
  • Target synonyms
    CDK8 protein kinase; cell division protein kinase 8; cyclin-dependent kinase 8; IDDHBA; K35; mediator complex subunit CDK8; mediator of RNA polymerase II transcription subunit CDK8; protein kinase K35
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49336
  • Protein Name
    Cyclin-dependent kinase 8
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDK8 using anti-CDK8 antibody (A01493-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK8 antigen affinity purified polyclonal antibody (Catalog # A01493-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK8 at approximately 53 kDa. The expected band size for CDK8 is at 53 kDa.
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