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ProductsBack/CDK9 Antibody (phospho-Thr186)

CDK9 Antibody (phospho-Thr186)

LS-C750617
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCDK9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDK9 Antibody (phospho-Thr186)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa/53kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 42kDa/53kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1025
  • Target name
    CDK9
  • Target description
    cyclin dependent kinase 9
  • Target synonyms
    C-2k, CDC2L4, CTK1, PITALRE, TAK, cyclin-dependent kinase 9, CDC2-related kinase, cell division cycle 2-like protein kinase 4, cell division protein kinase 9, serine/threonine protein kinase PITALRE, tat-associated kinase complex catalytic subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdk9 using anti-Cdk9 antibody (PB9537). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk9 antigen affinity purified polyclonal antibody (Catalog # PB9537) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk9 at approximately 43 kDa. The expected band size for Cdk9 is at 43 kDa.
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