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ProductsBack/CDKN1A / WAF1 / p21 Antibody (clone B459)

CDKN1A / WAF1 / p21 Antibody (clone B459)

LS-C170973
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCDKN1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDKN1A / WAF1 / p21 Antibody (clone B459)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:500 - 1:3000), IHC (1:50 - 1:100), WB (1:100 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B459
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1026
  • Target name
    CDKN1A
  • Target description
    cyclin dependent kinase inhibitor 1A
  • Target synonyms
    CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CIP1; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; P21; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P21/CDKN1A antigen affinity purified polyclonal antibody (Catalog # A00145-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P21/CDKN1A at approximately 21 kDa. The expected band size for P21/CDKN1A is at 21 kDa.
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