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Western Blot analysis of COS7 cells using p18 Polyclonal Antibody
Western Blot analysis of COS7 cells using p18 Polyclonal Antibody
Western Blot analysis of COS7 cells using p18 Polyclonal Antibody

CDKN2C Antibody

CSB-PA003620
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetCDKN2C
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDKN2C Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1031
  • Target name
    CDKN2C
  • Target description
    cyclin dependent kinase inhibitor 2C
  • Target synonyms
    CDK6 inhibitor p18; cyclin-dependent inhibitor; cyclin-dependent kinase 4 inhibitor C; cyclin-dependent kinase 6 inhibitor p18; cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4); INK4C; p18; p18-INK4C; p18-INK6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42773
  • Protein Name
    Cyclin-dependent kinase 4 inhibitor C
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDKN2C using anti-CDKN2C antibody (A03299-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDKN2C antigen affinity purified polyclonal antibody (Catalog # A03299-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDKN2C at approximately 18 kDa. The expected band size for CDKN2C is at 18 kDa.
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