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CDT1 Antibody

LS-C829973
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCDT1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CDT1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:30 - 1:150), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81620
  • Target name
    CDT1
  • Target description
    chromatin licensing and DNA replication factor 1
  • Target synonyms
    DNA replication factor Cdt1; Double parked, Drosophila, homolog of; DUP; RIS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: CDT1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa
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Figure 1. Western blot analysis of CDT1/DUP using anti-CDT1/DUP antibody (A01035-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1/DUP antigen affinity purified polyclonal antibody (Catalog # A01035-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDT1/DUP at approximately 60 kDa. The expected band size for CDT1/DUP is at 60 kDa.
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