CDw17 Antibody (Lactosylceramide)

Biorbyt

CDw17 Antibody (Lactosylceramide)

10

Optimal dilution of the HPV-16 L1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Flow Cytometry, ImmunoFluorescence

Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml FACS, IF

Research Use Only

Monoclonal

HO18.3G-6.F5

Unconjugated

Mouse

IgM

CD17 is an intermediate glycosphingolipid from the metabolism of higher gangliosides that localizes to sphingolipid-sterol rafts. CD17 is detectable in monocytes, granulocytes, basophils, platelets, a subset of peripheral B cells (CD19+) and tonsil dendritic cells. It is rapidly down regulated on activated granulocytes and is upregulated on IL-2 activated T lymphocytes. CD17 binds to bacteria and may function in phagocytosis. VEGF-treated endothelial cells can produce CD17, which can then mediate signaling toward PECAM-1 expression and angiogenesis. TNFa-induced astrogliosis (astrocyte proliferation and glial fibrillary acidic protein (GFAP) upregulation) in response to neuro-inflammation (i.e. spinal cord injury) causes an increase in intracellular levels of CD17. Aberrant levels of glycosphingolipids are a feature of cancer cells and may influence integrin clustering and internalization.

Human

-20°C,2°C to 8°C

12352203