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ProductsBack/CDX2 antibody [AFA-3]
WB analysis of 293 whole cell lysate using GTX04824 CDX2 antibody [AFA-3]. Dilution : 1:3000
WB analysis of 293 whole cell lysate using GTX04824 CDX2 antibody [AFA-3]. Dilution : 1:3000
WB analysis of 293 whole cell lysate using GTX04824 CDX2 antibody [AFA-3]. Dilution : 1:3000

CDX2 antibody [AFA-3]

GTX04824
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDX2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CDX2 antibody [AFA-3]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. FACS: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AFA-3
  • Conjugate
    Unconjugated
  • Gene ID1045
  • Target name
    CDX2
  • Target description
    caudal type homeobox 2
  • Target synonyms
    CDX-3, CDX2/AS, CDX3, homeobox protein CDX-2, caudal type homeobox transcription factor 2, caudal-type homeobox protein 2, homeobox protein miniCDX2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99626
  • Protein Name
    Homeobox protein CDX-2
  • Scientific Description
    This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDX2 using anti-CDX2 antibody (A00877-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human DLD1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDX2 antigen affinity purified polyclonal antibody (Catalog # A00877-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDX2 at approximately 45 kDa. The expected band size for CDX2 is at 33 kDa.
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