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CECR5 Antibody (YA2477, Rabbit)

HY-P82732
MedChem Express
TargetHDHD5
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CECR5 Antibody (YA2477, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID27440
  • Target name
    HDHD5
  • Target description
    haloacid dehalogenase like hydrolase domain containing 5
  • Target synonyms
    CECR5, haloacid dehalogenase-like hydrolase domain-containing 5, cat eye syndrome chromosome region, candidate 5, cat eye syndrome critical region protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CECR5 Antibody (YA2477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CECR5.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDHD5 using anti-HDHD5 antibody (A31869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD5 antigen affinity purified polyclonal antibody (Catalog # A31869-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDHD5 at approximately 50 kDa. The expected band size for HDHD5 is at 46 kDa.
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