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ProductsBack/CEND1 Recombinant Antibody, AbBy Fluor-647 Conjugated

CEND1 Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62057R-BF647
Bioss
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCEND1
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Overview

  • Supplier
    Bioss
  • Product Name
    CEND1 Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID51286
  • Target name
    CEND1
  • Target description
    cell cycle exit and neuronal differentiation 1
  • Target synonyms
    BM88, cell cycle exit and neuronal differentiation protein 1, BM88 antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N111
  • Protein Name
    Cell cycle exit and neuronal differentiation protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CEND1 using anti-CEND1 antibody (M13043). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEND1 antigen affinity purified monoclonal antibody (Catalog # M13043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEND1 at approximately 22 kDa. The expected band size for CEND1 is at 15 kDa.
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