
Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil.
CENPA antibody [3-19]
GTX13939
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityC. Elegans, Chicken, Human
TargetCENPA
Overview
- SupplierGeneTex
- Product NameCENPA antibody [3-19]
- Delivery Days Customer9
- Application Supplier NoteICC: Use at a concentration of 10 microg/ml. IHC: Use at a concentration of 5 microg/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 microg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3-19
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1058
- Target nameCENPA
- Target descriptioncentromere protein A
- Target synonymsCenH3; CENP-A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone; histone H3-like centromeric protein A
- HostMouse
- IsotypeIgG1
- Protein IDP49450
- Protein NameHistone H3-like centromeric protein A
- Scientific DescriptionCentromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]
- ReactivityC. Elegans, Chicken, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis.Read more
- PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size.Read more
- Kinetochore-microtubule attachment in human cells is regulated by the interaction of a conserved motif of Ska1 with EB1.Read more
- Beta Human Papillomavirus 8 E6 Induces Micronucleus Formation and Promotes Chromothripsis.Read more
- Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization. Pesenti ME et al., 2022 Jun 2, Mol CellRead more
- Super Enhancer-Mediated Upregulation of HJURP Promotes Growth and Survival of t(4;14)-Positive Multiple Myeloma.Read more
- Chromosome length and gene density contribute to micronuclear membrane stability. Mammel AE et al., 2022 Feb, Life Sci AllianceRead more
- CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer. Chen A et al., 2021 Feb, Cancer Commun (Lond)Read more
- Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80. Pesenti ME et al., 2018 Sep 20, Mol CellRead more
- Chmp4c is required for stable kinetochore-microtubule attachments. Petsalaki E et al., 2018 Dec, ChromosomaRead more