CENPA antibody [3-19]

GeneTex

CENPA antibody [3-19]

9

ICC: Use at a concentration of 10 microg/ml. IHC: Use at a concentration of 5 microg/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 microg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.

ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking

Research Use Only

Monoclonal

3-19

1 mg/ml

Unconjugated

CENPA

centromere protein A

CenH3; CENP-A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone; histone H3-like centromeric protein A

Mouse

IgG1

Histone H3-like centromeric protein A

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]

-20°C or -80°C,2°C to 8°C

12352203