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CEP110 antibody

70R-35787
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    CEP110 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA039402 antibody. Corresponding CCP110 RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of CP110 using anti-CP110 antibody (A05058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CP110 antigen affinity purified polyclonal antibody (Catalog # A05058-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CP110 at approximately 113 kDa. The expected band size for CP110 is at 113 kDa.
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