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Western Blot analysis of COLO cells using CEP135 Polyclonal Antibody
Western Blot analysis of COLO cells using CEP135 Polyclonal Antibody
Western Blot analysis of COLO cells using CEP135 Polyclonal Antibody

CEP135 Antibody

CSB-PA080046
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCEP135
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Overview

  • Supplier
    Cusabio
  • Product Name
    CEP135 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9662
  • Target name
    CEP135
  • Target description
    centrosomal protein 135
  • Target synonyms
    CEP4, KIAA0635, MCPH8, centrosomal protein of 135 kDa, centrosomal protein 135kDa, centrosomal protein 4, centrosome protein cep135
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ66GS9
  • Protein Name
    Centrosomal protein of 135 kDa
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CEP135 using anti-CEP135 antibody (A06361-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP135 antigen affinity purified polyclonal antibody (A06361-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP135 at approximately 133 kDa. The expected band size for CEP135 is at 133 kDa.
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