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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005271ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005271ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005271ESR2HU at dilution of 1:100

CFD Antibody

CSB-PA005271ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCFD
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Overview

  • Supplier
    Cusabio
  • Product Name
    CFD Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1675
  • Target name
    CFD
  • Target description
    complement factor D
  • Target synonyms
    ADIPSIN, ADN, DF, PFD, complement factor D, C3 convertase activator, D component of complement (adipsin), complement factor D preproprotein, properdin factor D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00746
  • Protein Name
    Complement factor D
  • Scientific Description
    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CFD using anti-CFD antibody (A01134-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFD antigen affinity purified polyclonal antibody (Catalog # A01134-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFD at approximately 45 kDa. The expected band size for CFD is at 27 kDa.
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