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ProductsBack/CFI / Complement Factor I Antibody

CFI / Complement Factor I Antibody

LS-C346093
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetCFI
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CFI / Complement Factor I Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 65kDa, while the observed MW by Western blot was 70kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 65kDa, while the observed MW by Western blot was 70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3426
  • Target name
    CFI
  • Target description
    complement factor I
  • Target synonyms
    AHUS3, ARMD13, C3BINA, C3b-INA, FI, IF, KAF, complement factor I, C3B/C4B inactivator, C3b-inactivator, Konglutinogen-activating factor, complement component I, complement control protein factor I, complement factor I heavy chain, light chain of factor I
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor I using anti-Factor I antibody (PB9935). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor I antigen affinity purified polyclonal antibody (Catalog # PB9935) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor I at approximately 75KD; 45KD. The expected band size for Factor I is at 66KD.
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