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Western blot All lanes: CFL2 antibody at 3.75microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse liver tissue Lane 3: Mouse lung tissue Lane 4: LO2 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19, 17 kDa Observed band size: 19 kDa
Western blot All lanes: CFL2 antibody at 3.75microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse liver tissue Lane 3: Mouse lung tissue Lane 4: LO2 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19, 17 kDa Observed band size: 19 kDa
Western blot All lanes: CFL2 antibody at 3.75microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse liver tissue Lane 3: Mouse lung tissue Lane 4: LO2 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19, 17 kDa Observed band size: 19 kDa

CFL2 Antibody

CSB-PA005281ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCFL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CFL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1073
  • Target name
    CFL2
  • Target description
    cofilin 2
  • Target synonyms
    NEM7, cofilin-2, cofilin 2 (muscle), nemaline myopathy type 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y281
  • Protein Name
    Cofilin-2
  • Scientific Description
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190). It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Cofilin 2 using anti-Cofilin 2 antibody (RP1107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, Lane 9: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin 2 antigen affinity purified polyclonal antibody (Catalog # RP1107) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin 2 at approximately 19 kDa. The expected band size for Cofilin 2 is at 19 kDa.
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Immunohistochemistry analysis in human skeletal muscle and skin tissues using HPA045599 antibody. Corresponding CFL2 RNA-seq data are presented for the same tissues.
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