IHC-P analysis of human colon carcinoma tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human pancreas tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22784/GTX22784_1105_IHC-P_w_23060620_365.webp "IHC-P analysis of human pancreas tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22784/GTX22784_1106_IHC-P_w_23060620_136.webp "IHC-P analysis of human tonsil tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![ICC/IF analysis of HeLa cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22784/GTX22784_433_ICC-IF_w_23060620_992.webp "ICC/IF analysis of HeLa cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of WiDr cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22784/GTX22784_434_ICC-IF_w_23060620_221.webp "ICC/IF analysis of WiDr cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22784/GTX22784_435_ICC-IF_w_23060620_779.webp "ICC/IF analysis of U251 cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human colon carcinoma tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
CFTR antibody [CF3]
GTX22784
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman, Mouse
TargetCFTR
Overview
- SupplierGeneTex
- Product NameCFTR antibody [CF3]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA.
- Application Supplier NoteWB: 1:500. ICC/IF: 1:500. IHC-P: 1:200. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDCF3
- ConjugateUnconjugated
- Gene ID1080
- Target nameCFTR
- Target descriptionCF transmembrane conductance regulator
- Target synonymsABC35; ABCC7; cAMP-dependent chloride channel; CF; CFTR/MRP; channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulating; cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); dJ760C5.1; MRP7; TNR-CFTR
- HostMouse
- IsotypeIgM
- Protein IDP13569
- Protein NameCystic fibrosis transmembrane conductance regulator
- Scientific DescriptionThis gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203