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ProductsBack/CHAF1B / CAF1 Antibody

CHAF1B / CAF1 Antibody

LS-C749087
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCHAF1B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CHAF1B / CAF1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 61kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 61kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8208
  • Target name
    CHAF1B
  • Target description
    chromatin assembly factor 1 subunit B
  • Target synonyms
    CAF1; CAF-1; CAF-1 subunit B; CAF1A; CAF1P60; CAF-I 60 kDa subunit; CAF-I p60; CAF-IP60; chromatin assembly factor 1 subunit B; chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit; M-phase phosphoprotein 7; MPHOSPH7; MPP7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p60 CAF1 using anti-p60 CAF1 antibody (M03959-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p60 CAF1 antigen affinity purified monoclonal antibody (Catalog # M03959-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p60 CAF1 at approximately 70 kDa. The expected band size for p60 CAF1 is at 61 kDa.
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