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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005325ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005325ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA005325ESR2HU at dilution of 1:100

CHD1 Antibody

CSB-PA005325ESR2HU
Cusabio
ApplicationsChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCHD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1105
  • Target name
    CHD1
  • Target description
    chromodomain helicase DNA binding protein 1
  • Target synonyms
    ATP-dependent helicase CHD1; CHD-1; chromodomain-helicase-DNA-binding protein 1; PILBOS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14646
  • Protein Name
    Chromodomain-helicase-DNA-binding protein 1
  • Scientific Description
    ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHD1 using anti-CHD1 antibody (A01175-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD1 antigen affinity purified polyclonal antibody (Catalog # A01175-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD1 at approximately 270 kDa. The expected band size for CHD1 is at 196 kDa.
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