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ProductsBack/CHD2 antibody [HL2066]

CHD2 antibody [HL2066]

GTX637970
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCHD2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CHD2 antibody [HL2066]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2066
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1106
  • Target name
    CHD2
  • Target description
    chromodomain helicase DNA binding protein 2
  • Target synonyms
    ATP-dependent helicase CHD2; CHD-2; chromodomain-helicase-DNA-binding protein 2; DEE94; EEOC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14647
  • Protein Name
    Chromodomain-helicase-DNA-binding protein 2
  • Scientific Description
    The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHD2 using anti-CHD2 antibody (A04079-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD2 antigen affinity purified polyclonal antibody (Catalog # A04079-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD2 at approximately 250 kDa. The expected band size for CHD2 is at 211 kDa.
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